Abstrait

Validated method for the simultaneous estimation of domperidone and lansoprazole in pure and pharmaceutical dosage formbyRP-HPLC

M.VijeyAanandhi, N.Thiyagarajan, S.Sesha Panindra, P.Shanmugasundaram, R.Sujatha

A novel simple, fast, accurate and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of domperidone and lansoprazole in capsule formulation. In thismethod, the chromatographic resolution was carried out on a Hypersil ODS C18 (2504.6 mm) column by using a mobile phase containing phosphate buffer and acetonitrile (pH 7.4) in the ratio of 60:40, in an isocratic elution at a flow rate of 1.5 ml/ min. The detection of eluents was done at 302 nm. The retention time for domperidone and lansoprazole were 5.49 min and 2.10 min respectively. Linearity for domperidone was found to be in the range 40-100g/ml and 60-140g/ml for lansoprazole respectively.