Abstrait
Spectrophotometric Method for the Determination of Stavudine Residues in Swab Samples
Rohit Dutt, Vandana Dutt, Kumar Gaurav, D. P. Pathak and Gajendra Singh
The objective of present investigation was to develop a simple, cheap, fast, accurate, sensitive and precise UV spectrophotometric method that can be used for the determination of stavudine residues in swab samples. The drug standard solution was scanned over entire UV range and a wavelength of 266
nm was selected for analysis. The swabbing procedure was optimized in order to obtain a suitable recovery from stainless steel surface using Tex Wipe Polyurethane swab stick. The drug solution showed sufficient absorption at 266 nm and low quantities of stavudine may be detected correctly. A
mean recovery of 99 % was obtained, when swabs dipped in water were used. Beer’s law is valid in concentration range of 1-20 mcg/mL. The results were found to be satisfactory with good stability up to 48 hours at ambient temperature.
