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Simultaneous Estimation of Perindopril and Amlodipine in Combined Dosage form by RP-HPLC Method

V. Bhaskara Raju and A. Lakshmana Rao

An accurate and precise HPLC method was developed for the simultaneous determination of perindopril and amlodipine. Separation of the drugs was achieved on a reverse phase C18 column using mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 65 : 35 v/v. The flow rate was 0.6 mL/min and the detection wavelength was 237 nm. The linearity was observed in the range of 10-50 μg/mL for amlodipine and 200-1000 μg/mL for perindopril. The proposed method was validated for its linearity, accuracy, precision and robustness. The proposed method can be employed to estimate the drug contents in marketed formulations.