Abstrait

Simultaneous determination of several angiotensin-II receptor antagonists by liquid chromatography

S.G.Hiriyanna, K.Basavaiah, V.Dhayanithi, A.Bindu, Sudhaker Pujari, Hari N.Pati

We optimized a simple rapid and elegantHPLCmethod for the simultaneous quantification of six angiotensin-II-receptor antagonists: Losartan, Olmesartan, Irbesartan,Valsartan, Telmisartan and Candesartan. The quantification is carried out using HPLCwith UV detection. Combination of the studied parameters permitted the separation of the sixARA-IIs, which was best carried out using 10mM Potassium dihydrogen phosphate buffer (pH 3.5) with acetonitrile,methanol and commercially available ODS stationary phase column. The main feature of the method developed is that it can separate and unequivocally quantify chemically similar compounds in a single run utilizing a gradient elution profile. The retention time of Losartan, Olmesartan, Irbesartan, Valsartan, Telmisartan and Candesartan were 9.0, 10.3, 11.5, 12.0, 13.4 and 19.9min, respectively. Some parameters (linearity, limit of detection and quantitation, Stability of solution, Precision and accuracy) were validated. The method was accurat, precise and suitable for the intended purpose.