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Seminal sulfhydryl oxidase: Appropriate fluorometric assay for the examination of spermatozoa disorders

Mahmoud Hussein Hadwan, Lamia A.Almashhedy, Abdul Razzaq S.Alsalman

A Fluorometric assay for serum sulfhydryl oxidase activity that could be used as a back-up to current tests in the differential diagnosis of spermatozoa disorders is described. The assay is based on the H2O2- dependent oxidation of dichlorofluorescein catalyzed by peroxidase mimetic (amixture of hematin (HT) and hemoglobin (HB)). Themethod is sensitive, precise (CV below 5.25%), and linear up to 200 µmol/l. The analytical recovery of the present method is estimated. The comparison with the colorimetricmethod gave good correlation (r = 0.9929). Themethod is applied to the measurement of the SHO activity in seminal plasma of fertile men andAsthenzoospermic patients. Reference values for seminal sulfhydryl oxidase activities determined with the present method on 60 healthy persons are 68.27 ± 4.03 nmol/mg protein.min.