Abstrait

Sample preparation and preconcentration for determination of chlorophenols in water and sediment samples

W.Kanchanamayoon, S.Pumporn, J.Tantidechamongkorn, A.Bamrungsook

Chlorophenol compounds are used in many industries including the fabric, dye and pesticide industries. Most of the compounds are toxic to health and often contaminate wastewater and sediment. A sample preparation was investigated to optimize the extraction of phenol, 2-chlorophenol, 2,6- dichlorophenol, 2,3,6-trichlorophenol and 2,3,4,6,-tetrachlorophenol inwater and sediment samples. The samples were analyzed by high performance liquid chromatograph. ENVI-18 solid-phase was suitable for extraction of chlorophenols in water samples using 5 mL of methanol:acetonitrile as an eluting solvent. Recoveries were in the range of 81.98-103.75 percent. Optimized solid-phase extraction was applied for extraction of chlorophenols in natural water and bottled drinking water. Analysis was by high performance liquid chromatography. The results showed that no chlorophenol contamination was found in all water samples. Chlorophenols in sediment samples were achieved with solvent extraction using acetonitrile:acetone (1:1) as an extracting solvent with a 30 min extraction time. Recoveries ranged from93.85-109.39 percentwith a relative standard deviation (RSD) 0.90-3.75%. The optimumconditionswere applied for extraction of chlorophenols in 6 sediment samples and determined by high performance liquid chromatography. The results showed that no contamination was found in all samples.