Abstrait

Preparation, characterization and in vitro evaluation of rifampicin loaded biodegradable microsphere

Atanu Kumar Behera B.B.Barik

The aim of this study was to prepare Rifampicin-loaded Polycaprolactone (PCL) microspheres by emulsion solvent evaporation technique with different drug-to-polymer ratios (1:5), (1:10), (1:15), (1:20) and (1:25), characterize and evaluate the in vitro performance. The microspheres were characterized for particle size, surface morphology, drug excipient compatibility, percentage yield, drug entrapment, and in vitro release kinetics. Of five formulations prepared,MP 4, i.e., 1:20 (drug-polymer) ratio was selected as the optimized formulation based on particle size, particle shape, and the release behavior. The size of microspheres was found to be ranging from 1.9 to 2.3 ìm. The shape of microspheres was found to be spherical by SEM. Among the four formulations, MP 4 (1:20) showed a maximumpercentage yield of 79%± 3.4%. There was no interaction between drug and polymer by FT-IR study. In the in vitro release study, formulation MP 4 showed 75.6 % drug release and was found to be sustained for 15 days. The microsphere formulations were able to sustain the release of drug in vitro.