Biochimie : une revue indienne
ISSN (PRINT): 0974-7427

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New Spectrophotometric Method for the Estimation of Pantoprazole in Bulk and Pharmaceutical Formulation

N. V. Pimpodkar, R. S. Nalawade , B. S. Kuchekar, N. S. Mahajan and R. L. Jadhav


A simple, sensitive, rapid and accurate colorimetric method has been developed for the estimation of pantoprazole in bulk and pharmaceutical dosage forms. This method is based on oxidative coupling reaction of pantoprazole with 3-methyl-2-benzathiazoline hydrazone (MBTH) in presence of ferric ammonium sulphate producing orange red colored chromogen, which exhibits absorption maximum at 504 nm. It obeyed Beer’s law in concentration range of 10-90 μg/mL. The results obtained with the proposed method are in good agreement with labeled amounts, when marketed pharmaceutical preparations are analyzed. Results obtained are statistically validated and found to be reproducible.


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