Abstrait

Highly Sensitive Square Wave Voltammetric Technique for Determination of Nepafenac in Bulk Powder, Pharmaceutical Dosage Form and Blood Serum

Daabees HG, Talaat W, El-Shal M and Ahmed K

It introduces a square wave voltammetric (SQV) technique for the determination of Nepafenac in pharmaceutical dosage form and human serum with accepted accuracy and precision to be applied for routine analysis of nepafenac. Carbon paste (CPE) electrode is modified with carbon multiwalled nanotube (CMWNT), 1-n-butyl-3-methylpyridinium hexafluorophosphate ion crystal (BMH) and sodium dodecyl sulfate (SDS), [(CMWNT-BMH-SDS) electrode], for determination of NPF in bulk powder, pharmaceutical dosage form and biological fluid. The proposed voltammetric technique was established on electro-oxidation of nepafenac at (CMWNT-BMH-SDS) electrode in 0.04 M B-R electrolyte with pH=7.0. All procedures were performed in 0.04 M B-R electrolyte with pH=7.0. Experimental and instrumental parameters for quantitative determination were optimized. The peak current concentration relationship was rectilinear over the range 0.333-6.327 μg/mL. Detection and quantification limits were found to be 0.081 and 0.272 μg/ml, respectively. Precision and accuracy for the suggested method were checked by recovery studies with acceptable repeatability and reproducibility, the suggested method was applied successfully to the dosage form and human serum. The suggested electrode was considerably stable. Electrode preparation is easy, simple and available surface renewal. The modified electrode demonstrated higher selectivity towards nepafenac in routine analysis.