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Functional evaluation of ToxA promoter in Trichoderma reeseiRut-C30

Ortiz E.Gast�������³n, Albert�������³ Edgardo, BlascoMart�������­n

In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vectorwas employed to transformT. reesei Rut- C30. The transformants obtained were evaluated bymeans of fluorescence microscopy, fluorometry, dot blot andWestern blot analysis. The lowlevels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30.