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Estimation of flavonoid-luteolin in different plant parts of Bacopa monnieri (L.) Wettst. by using HPTLC method

Anju Varshney, Sunita Shailajan, Naresh Chandra

Bacopa monnieri (L.) Wettst., commonly known as Brahmi or Jalanimba in India is distributed throughout the warmer regions of the world.Asimple, sensitive and accurate HPTLCmethod has been developed for quantitation of a flavonoid Luteolin from micropropagated, native and cultivated plant parts (roots, leaves and stem) of Bacopa monnieri collected from two different locations (Bhayander and Chembur) of Maharashtra. Samples were extracted withmethanol and spotted onHPTLC silica gel 60 F254 pre-coated plates using automatic sample spotter (Linomat IV) with toluene: ethyl acetate: formic acid (3:3:0.8; v/v/v). Spectra of Luteolinwere comparable only with the leaf collected from Bhayander and stem collected from Chembur. Densitometric evaluation of the plates was performed using deuteriumlamp withCamag Scanner II equippedwithWinCats 3 software at 254 nm. Luteolin response was linear over the range 20 µg/mL to 150 µg/mL. Method was validated as per ICH guidelines.