Abstrait

Enzymatic synthesis of levan polysaccharide by Bacillusm licheniformis levansucrase

Imen Dahech, Rania Bredai, Karima Srih

Levan can be synthesized by the microorganism Bacillus licheniformis. The enzymatic synthesis of levan was carried out in erlenmeyers flasks and the influence of synthesis conditions was studied. The results show that the enzyme can produce an important amount of levan up to 80 g/l of levan when a concentration of 300 g/l of sucrose was used at pH 8 at 50°C for a period of 18h. The obtained results are also importantmaking levan different from the previously reported levans and can be successfully used in industrial applications.