Abstrait

Endoglucanase, Exo-glucanase and Alpha-glucosidase Activity the Combination of Crude Enzyme from Trichoderma reesei and Aspergillus niger at Different Temperatures and pH

Bambang Dwi Argo, Agustin Krisna Wardani, Elok Zubaidah and Sri Winarsih

The purpose of this study was to compare the activity of the crude cellulase enzyme produced by Trichoderma reesei and Aspergillus niger and the various combination of two crude enzymewith a ratio of Trichoderma reesei and Aspergillus niger (0/1, 1/0; 1/1; 1/2, 2 / 1; 1/3; 3/1 v / v) at different pH and temperature. The results showed that the highest exo-glucanase activity (FP ase), endo-glucanase activity (CMC ase) and Alpha-glucosidase activity of combined enzyme of Trichoderma reesei :Aspergillus niger in sequence (2 : 1) at pH5 is 1.002 IU/ml; (1 : 3) at pH5 is 2.90 IU/ml and (1 : 3) at pH4.5, generated 0.316 IU/ml and three of those activity reached the highest level at temperatures of 50oC.