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Determination of ascorbic acid in human’s blood serum using electrochemically activated pencil graphite electrode

Shokuhi Rad, M.Ardjmand


The intake of sufficient amount of vitamin C (ascorbic acid) in the daily nutrition of human being is necessary to have a healthy life. Therefore, determining the exact amount of this supplement in the HumanÂ’s blood samples is one of the important measures for the assessment of healthy life. After improving the catalytic activity, the pencil graphite electrode was used to determine the amount of vitamin C in the humanÂ’s blood plasma samples. The obtained results show that the anodic peak current for ascorbic acid is linear for the concentration range of 10-6 to 10-4M having the correlation coefficient of 0.9998. The obtained detection limit forAAdetermination using amperometry technique was 1.18ï‚´10-6M.


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