Abstrait

Biochemical effects of Cleome droserifolia on hepatic dysfunction in alloxan-induced Diabetes mellitus in male albino rats

M.A.Nagy

Background: Cleome droserifolia is considered to have protective effects against several diseases. The hepatic dysfunction associated with Diabetes mellitus (DM) has been reported and was found to be associated with oxidative damage. This studywas conducted to evaluate the role of Cleome droserifolia to protect against alloxan -induced liver dysfunction in rats. Method:Alloxanwas administered i.p. in a single dose (150mg/kg) to adult male rats.Alloxan-induced diabetic rats were orally administeredMethanol water extract of Cleome droserifolia (MWCD) (0.31 g /kg body weight) daily in accordance to (Nicola et al., 1996) of rats daily for 30 days after alloxan injection). Result:Alloxan administration to rats resulted in significant elevation of serum transaminases (sALT and sAST), depletion of hepatic reduced glutathione (GSH), catalase (CAT) and glutathione peroxidase (GPx), elevation of lipid peroxides (LPO) expressed asmalondialdehyde (MDA). Significant rises in liver tumor necrosis factor-alpha (TNF-) and caspase-3 levelswere noticed in alloxan-induced diabetic. Treatment of the alloxan-induced diabetic rats with MWCD significantly prevented the elevations of sALT and sAST, inhibited depletion of hepatic GSH, GPx, CAT and inhibited MDA accumulation. Furthermore, MWCD had normalized serum total proteins and hepatic CAT, TNF-  and caspase-3 levels of alloxan-induced diabetic rats In addition, MWCD prevented the alloxan - induced apoptosis and liver injury as indicated by the liver histopathological analysis. Results showed significant correlation in either alloxanMWCD group between TNF-  and each of serumALT,AST and liver GPX, CAT, GSH,MDAand caspase-3 levels.Conclusion: our data indicate thatMWCD protects against alloxan -induced liver injury in rats through antioxidant, anti-inflammatory and antiapoptotic mechanisms. However, further merit investigations are needed to verify these results and to assess the efficacy ofMWCD therapy to counteract the liver dysfunction and oxidative stress status.