Abstrait

Application of area under curve technique forUV-spctrophotometric determination of benazepril in bulk and tablet formulation

Kishor V.Pachpute, Amod S.Patil, Harshal D.Mahajan, Bhushan J.Mali, Bhupendra L.Deore

Novel, accurate, precise and economical UV- spectrophometric method has been developed for estimation of Benazepril hydrochloride in bulk and tablets using area under curve (AUC) technique. The method is based on calculation of area under curve for analysis of Benazepril hydrochloride in the wavelength range of 228.40 - 252.20 nm.Methanol was used as solvent for preparation of standard and sample solutions. Calibration curves were plotted by using instrumental response at selected wavelength and concentrations of analyte in the solution. The drug obeyed BeerÂ’s law in the concentration range of 2 - 12 µg/mL. The proposed methodcan be used for routine analysis since it is rapid, simple, accurate and also sensitive. The results obtained are reproducible with a % relative standard deviation less than 2%. This method was validated for accuracy, precision, repeatability, sensitivity and ruggedness as per ICHguidelines.