Abstrait

A new method for determination of fenoprofen in urine and human plasma by differential pulse adsorptive stripping voltammetry

ShahryarAbbasi, Saeid Zadkhast, HosseinKhani

A novel selective and sensitive electrochemical method is developed for determination of fenoprofen by adsorptive stripping voltammetry (DPAdSV). Fenoprofen gave well resolved diffusion controlled cathodic peak at -0.989 V vs. Ag/AgCl reference electrode in phosphate buffer. Optimal conditions were obtained at pH 4.4, accumulation potential 0.15 V, accumulation time of 40 s, and scan rate of 120 mV/s. Under the optimized conditions, linear calibration curves were established for the concentration of fenoprofen in the range of 0.418-52.26 ì g/ml, with detection limit of 0.081ìg/ml. The relative standard deviation of the methodfor 10 runs at 0.418 and 4.18 µg/m lfenoprofen was 3.18%, 2.49%,, respectively. The method was applied to the determination of fenoprofen in various biological samples with satisfactory results.